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  (Nucleoside Chemistry)


Other Names:


Methylidyne trichloride

General Information:


CAS Number: 67-66-3

Molecular Weight: 119.38 g/mol

Appearance: Colorless liquid

Chemical Formula: CHCl3

Melting Point: -63 C

Boiling Point: 61 C

Density: 1.489 g/mL

Chloroform (CHCl3) is a relatively common organic solvent. In most organic chemistry labs, however, methylene chloride is used as a substitute. Deuterated chloroform (CDCl3) is one of the most common NMR solvents.

Common Uses:

Solvent in bromination reactions

Solvent in manganese dioxide (MnO2) oxidations

Solvent for silica gel chromatography

Procedure excerpt:

…The crude residue was purified by silica gel column chromatography (0-1% MeOH/CHCl3) to provide the product as a light yellow solid…

Solvent for extractions

Procedure excerpt:

…was concentrated to a residue and partitioned between H2O and CHCl3. The layers were separated and the aq layer was further extracted with CHCl3 (4x)…


Chloroform (CHCl3) is dangerous due to its ability to depress the central nervous system. In fact, chloroform was once commonly used as an anesthetic for just these CNS depressing effects. A fatal oral dose can be a small as 10 mL. Care should be taken when working with large amounts of chloroform.


1) Patent Reference: WO2014177977, page 64, (6.0 MB)

2) Patent Reference: WO2015140133, page 90, (11.7 MB)

3) Patent Reference: WO2011014535, page 46, (17.4 MB)

4) Patent Reference: WO2010104899, page 155, (6.7 MB)

5) Wikipedia: Chloroform (link)

6) Chloroform (link)